FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n01_A2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n01_A2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26664501
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG1407660.5279153733272564TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG179350.038.16374645
AGTCACG192450.038.12663728
CTCGTAT183550.037.98921644
ACGTCTG198900.037.86001215
ACGTCCG193200.037.76687232
CGTCTGA199200.037.7013416
GTCACGT199850.036.7374129
GTCCGCA201900.035.99595334
CCGCACA203200.035.6217836
ATCGGAA214200.035.088582
TCACGTC208950.035.0618830
TCGGAAG216450.034.899263
CACACGT215850.034.8765612
CACGTCT219800.034.4033414
CGCACAT211450.033.7956737
ACACGTC225050.033.5707813
TCCGCAC222750.032.7780935
CGTCCGC226200.032.41644333
TCTCGTA217400.032.07421543
CAGTCAC231400.032.0494927
Produced by FastQC (version 0.11.5)