FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n01_A2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n01_A2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29680932
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG59750.026.09497615
CGTCTGA62750.024.56056816
GTCCGCA62250.023.92756536
CTCGTAT56400.023.65760844
TCGTATG59550.021.53715545
CGCATCT71450.020.50077839
CACGTCT79400.020.20368414
TCGGAAG78650.020.1987923
ATCGGAA77900.020.1917062
ACCCGTC78550.019.62084432
TCCGCAT79150.018.87543737
CACACGT84050.018.81824712
CCGTCCG94000.017.06644234
TCACCCG90050.017.04100230
ACACGTC96350.016.53267513
CCCGTCC96700.016.1940733
GTCACCC96350.015.88009729
CAGTCAC100950.015.80286627
TCTCGTA87350.015.53279543
CGTCCGC106200.015.12694935
Produced by FastQC (version 0.11.5)