FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n01_A2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n01_A2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23135849
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA301210.13019189397372016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG34450.025.3396215
CGTCTGA37250.023.55569616
CTCGTAT31150.018.4919944
ATCGGAA48950.017.9748862
CACGTCT49600.017.826614
TCGTATG30250.017.6288845
TCGGAAG50350.017.5188713
CACACGT52750.017.2312412
GTCAGAA50950.017.0461436
GTCACAT55100.016.49779329
TGTCAGA56250.016.03987735
AGTCACA57850.015.32459428
CAGTCAC65550.014.27962227
ACACGTC65400.014.27670513
TCACATG70100.013.12788730
CCAGTCA69650.013.01907226
AGCACAC75900.012.390326510
AATCTCG59250.012.34239941
ACATGTC75100.012.31322232
CACATGT77450.012.25937631
Produced by FastQC (version 0.11.5)