FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n01_A2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n01_A2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29849530
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG714570.23939070397423343TruSeq Adapter, Index 14 (97% over 44bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA341530.11441721192930006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT111350.035.38210739
AGTTCCG117300.034.35371834
TCCGTAT116050.034.2583837
CCGTATC116500.034.048838
CTCGTAT108300.033.4909944
TCGTATG105350.033.04054345
TTCCGTA127900.031.24259836
GTTCCGT130200.030.81150235
TATCTCG128150.029.5500641
ACGTCTG140950.029.32271815
CGTCTGA141750.029.06199516
GTATCTC148550.026.65801440
GTCACAG154350.026.41542229
TCTCGTA142100.025.98399543
ACAGTTC156850.025.89193332
CACGTCT161200.025.764814
TCACAGT160050.025.48842630
CACACGT166300.025.15053612
CACAGTT164250.024.9041231
AGTCACA164650.024.87228428
Produced by FastQC (version 0.11.5)