FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n01_A2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n01_A2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25146429
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA331500.13182786311328737No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271180.10784036174679115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG43950.025.90312215
CGTCTGA47050.023.95733816
CTCGTAT41800.023.90068444
TCGTATG44350.022.88160945
ATCGGAA58250.019.508552
TCGGAAG60700.018.9428143
CACGTCT62400.018.85721614
CACAGTC60050.018.80911831
CACACGT63150.018.34823612
GTCACAG66550.017.17592629
ACACGTC72150.016.52722713
TCACAGT71450.016.06077230
AGTCAAC71950.015.66704934
AGTCACA73400.015.66496228
AATCTCG68400.015.65865641
CAGTCAC76700.015.343019527
TCTCGTA68500.014.97883143
CCAGTCA83450.014.07500926
ACAGTCA87700.014.03320432
AGCACAC86400.013.74889210
Produced by FastQC (version 0.11.5)