FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17185672
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT5100.029.11504437
GTAGATC5150.028.3970932
CACTCTT7850.028.11312
TAGATCT5400.027.49762233
GTGTAGA5450.026.00904530
GAGTGTA5500.025.77297428
AGTGTAG5600.025.31259529
TGTAGAT6300.022.85689531
TTCCCTA4150.022.2241468
TCGGTGG6850.021.02430339
GATCTCG7600.020.42253535
GTAGGGA7300.020.34159319
TCGTGTA7300.020.34094215
TGTAGGG7350.020.20321518
GTGTAGG7400.020.0667117
CTCGGTG7700.019.87170838
AGATCTC7800.019.32108934
TAGGGAA7750.019.16063720
TCCCTAC4550.018.787079
TTTCCCT7150.017.3042817
Produced by FastQC (version 0.11.5)