FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n02_N2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n02_N2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27696555
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26700.012.0608422
GTAGAAC35200.010.86408311
CACTCGT51800.010.42290335
CTCTATA56650.09.93041245
GCGTGAC37000.09.8006811
ACTCGTG56100.09.6647836
TCACTCG56850.09.37786334
GTACTCG52700.09.3132874
TCAGGTA42800.09.2506157
GGAAAAT122600.08.8366311
TACTCGA54500.08.7551715
CTCGAAT26450.08.6005811
AATCGAA52400.08.5047035
AAAATCC87700.08.4984483
GCGGAAA74850.08.4556431
GGTAGAA48200.08.26073310
GAATCGA43450.08.1351764
GTGACTC47100.08.0793623
TAGAACA47950.08.06899712
TCGTGAG69350.07.94922638
Produced by FastQC (version 0.11.5)