FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n02_N2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n02_N2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27853042
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC43550.010.02067811
AAAATCC92300.09.9776963
GGAAAAT133800.09.9486321
CGTGACT19300.09.6843752
GTACTCG43650.09.5419614
TCAGGTA51650.09.3205557
AATCGAA53050.09.2488855
GAATCGA42550.09.1007894
CTCGAAT26100.08.6296171
TACTCGA49100.08.6177645
AAATCCT103350.08.1908234
GGTAGAA55250.08.18372810
AAAAATC84900.08.0898962
CACTCGT37550.08.08770535
AGTACTC54250.08.0521283
TAGAACA54300.08.03661112
ACTCGTG39350.08.00413436
CAGGTAG56900.07.9464578
GCGGAAA50100.07.77750641
CGCGAAT30400.07.772135741
Produced by FastQC (version 0.11.5)