FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n02_N2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n02_N2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30060966
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC42100.010.09850611
TCAGGTA52200.09.13596257
GGAAAAT129350.08.5145281
CAGGTAG50500.08.4634258
GGTAGAA58250.07.800778410
CACTCGT39550.07.67867735
GAATTGA90500.07.61227664
TAGAACA57750.07.59538712
AGAACGG179000.07.43418363
GCGGAAA57600.07.4293231
GGGAAAT76450.07.4240551
GAATCGA55300.07.24663734
GGGGAAT55400.07.19584271
AATCGAA63200.07.05113365
TCACTCG44700.07.0454534
GGAAGAT199650.06.96039631
ATCTAGC44300.06.95725066
CGTGACT21800.06.9208672
AAAATCC90350.06.90319973
AAGAACG232650.06.89158542
Produced by FastQC (version 0.11.5)