FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n02_N2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n02_N2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26769666
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC37350.010.65999911
CACTCGT46050.010.16058635
TCAGGTA44200.09.5680247
ACTCGTG49700.09.50567236
CGTGACT20300.09.3185962
TCACTCG50700.09.09521834
GGAAAAT110150.08.8543831
GCGGAAA64450.08.7371951
CAGGTAG47500.08.6190548
CTCTATA55250.08.51259945
TAGAACA48700.08.17540112
GAATCGA42600.08.0861974
GAATTGA69450.07.9749014
TCGTGAG61650.07.847329638
AAAATCC81250.07.787723
AAAAATC74350.07.6328512
CTATAAG26900.07.6164324
GGTAGAA50850.07.520365710
AGAACGG168200.07.45687063
GAAAAAT150900.07.4484561
Produced by FastQC (version 0.11.5)