FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n02_N2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n02_N2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27167745
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24250.012.44321252
GTAGAAC38600.012.238234511
TCAGGTA47350.010.6896757
GCGTGAC35400.09.289141
CTCGAAT25950.09.1133451
TAGAACA52400.09.10084512
GGTAGAA52750.09.04076110
GTACTCG48100.08.9868194
CACTCGT44400.08.91726535
GGAAAAT121800.08.9130361
CAGGTAG55150.08.8921458
AAAATCC83400.08.7744193
GAATCGA42450.08.6979324
AATTGAC42650.08.2322055
TACTCGA53200.08.1650025
GAATTGA70900.08.1608754
TCACTCG47800.08.0943634
AATCGAA50000.07.92233755
CTCTATA50350.07.821370645
ACTCGTG50050.07.821202336
Produced by FastQC (version 0.11.5)