FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n02_N2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n02_N2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29211793
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC37350.010.29880311
CACTCGT52650.09.14344935
CTCGAAT27850.08.8961
TCAGGTA48900.08.5106357
GGAAAAT133400.08.4250411
ACTCGTG56800.08.39689336
AATCGAA59450.08.3290615
CTCTATA59050.08.30738545
GAATCGA44000.08.2380874
TCACTCG57150.08.1082934
GCGGAAA72600.08.0040611
CGTGACT26250.07.89242842
GTACTCG46400.07.8119794
GAATTGA83250.07.78863574
GGTAGAA53000.07.76717610
AAAATCC89250.07.69491053
AGAACGG188500.07.6569993
GGGAAAT67250.07.6025771
CAGGTAG53650.07.50538168
TAGAACA53800.07.400589512
Produced by FastQC (version 0.11.5)