FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n02_N2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n02_N2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24009733
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT20050.010.8941122
CTCGAAT23300.010.342851
GTAGAAC38500.010.22494811
TCAGGTA45600.09.4223587
GTACTCG46850.09.3224234
AAAATCC74050.09.1524793
GGAAAAT105500.08.9662161
GCGTGAC30350.08.53397751
AATTGAC37200.08.4702275
GAATTGA59650.08.4542344
TACTCGA50950.08.4372165
AATCGAA47050.08.3233785
GGTAGAA49500.08.225471510
CAGGTAG50350.08.17598158
GAATCGA41100.08.1617114
TAGAACA48100.08.090507512
CACTCGT35650.07.82458535
AGTACTC60850.07.73362453
AAATCCT82450.07.61816264
ATTGACC39700.07.59347536
Produced by FastQC (version 0.11.5)