FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n02_N2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n02_N2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35324297
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC41750.08.99797311
AATCGAA72900.08.2741115
AAAATCC113200.08.1353833
CTCGAAT37750.08.0545911
GGAAAAT176300.07.84409331
CGTGACT28600.07.79492862
GAATCGA54750.07.6484484
CACTCGT50100.07.633238335
CAATCGA70300.07.58992534
GAATTGA107150.07.3749564
TCAGGTA55150.07.30145557
ATCTAGC60550.07.2824216
AATTGAC65700.07.2282175
GAAAAAT194000.07.174861
CCGAAAT38250.07.12493041
TCACTCG53600.07.09257934
ACTCGTG53900.07.053771536
AAATCCT121250.07.0372344
GCGGAAA73650.07.0030851
CAGGTAG59150.06.9977088
Produced by FastQC (version 0.11.5)