FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n02_N2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n02_N2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25992434
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23400.09.8157172
GGAAAAT117850.08.5999491
CACTCGT47550.08.27933635
GTAGAAC32750.08.10513711
AATTGAC44950.08.0613565
GAATTGA75050.07.9793944
GGGAAAT62100.07.9063831
ACTCGTG50600.07.825135736
TCACTCG50050.07.73070334
CTCTATA50750.07.71489245
GCGTGAC34900.07.67952731
ATTGACC44450.07.3389436
GCGGAAA65850.07.2509191
GTACTCG39800.07.24045564
AAAATCC80400.07.22536043
TCAGGTA42850.07.19238147
CTCGAAT27250.07.19060951
AGAACGG140250.07.16024453
GAATCGA40400.07.13292364
AATCGAA59250.06.98942385
Produced by FastQC (version 0.11.5)