FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17185672
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG4350.033.07628315
CGTCTGA4450.032.8387716
CACACGT5150.027.938312
CACGTCT5550.025.92465414
GAGACCG6000.024.77611731
ACCGATC5650.024.6742434
ACACGTC6000.024.35499813
CCGAGAC6150.023.822629
AGACCGA6350.023.0507632
AGCACAC6900.022.4785910
CAGTCAC4700.022.06020727
GCACACG7500.019.7838111
CGAGACC7650.019.44472130
CCAGTCA5600.019.31980926
CCGATCT7850.018.04528835
ATGCCGT8000.018.01211545
TCTAGTC750.001118221717.982827
GGAATGC9250.017.78233723
AGGAATG9400.017.24242822
CTCGTAT8450.016.51916940
Produced by FastQC (version 0.11.5)