FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n01_N2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n01_N2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27696555
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313420.11316208820916537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG46350.025.09612715
CGTAATC44150.024.9714138
CCGTACG47600.023.63380633
CGTCTGA49450.023.38640816
ACCGTAC53100.022.1604132
CTCGTAT45000.021.95003344
TCGTATG44550.021.46467845
CACCGTA55950.020.22798331
TCGGAAG63000.018.6456783
ATCGGAA64700.017.8435362
CACGTCT68300.017.689514
CACACGT68200.017.48454312
GTAATCT66600.016.99320639
TACGTAA66850.016.7612536
ACGTAAT66600.016.553937
CAGTCAC70700.016.32640327
AATCTCG73900.015.31463741
AGTCACC81700.014.623960528
ACACGTC84850.014.4777813
TAATCTC79500.014.29247440
Produced by FastQC (version 0.11.5)