FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n01_N2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n01_N2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27853042
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG28650.028.82184633
GTTTCGG38050.022.35241734
ACGTCTG39500.021.98640315
AGTCACG39500.021.2475328
CGTCTGA42700.020.44412616
TTCGGAA40600.020.2279536
TTTCGGA43700.019.10190635
ACGTTTC46000.018.34193832
CTCGTAT34750.018.1292144
TCGGAAT49950.016.71177737
TCGTATG36600.016.53662345
GTCACGT52200.016.2505329
CACACGT57300.015.47043212
TCGGAAG57900.015.2357913
ATCGGAA57500.014.9901642
CGGAATC57050.014.35585938
CACGTCT63950.014.10794914
GGAATCT59450.013.66289439
CACGTTT65000.013.15408631
ACACGTC74450.012.39022613
Produced by FastQC (version 0.11.5)