FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n01_N2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n01_N2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30060966
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG58900.025.86063215
AGTCACG58250.025.64840328
CGTCTGA63750.023.89323816
CTCGTAT55000.023.03196544
CACGTGG64650.022.96990831
GGCCTTA66500.021.99254836
TCGTATG54900.021.92636945
GTCACGT68350.021.85836629
TATCTCG67600.020.40323641
GCCTTAT74150.019.75393737
CACACGT79750.019.46620412
ATCGGAA77200.019.4433172
CACGTCT79700.019.36546714
TCACGTG78800.019.10234330
ACGTGGC80600.018.59131232
TCGGAAG81100.018.5911253
ACACGTC95500.016.67984613
CAGTCAC93850.016.49456627
CCTTATC90300.016.29567738
CTTATCT93100.016.0232239
Produced by FastQC (version 0.11.5)