FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n01_N2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n01_N2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26769666
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG45300.026.47410828
ACGTCTG49350.025.16623515
CGTCTGA49250.024.94323316
TCGTATG48050.024.06845745
CGTGAAA50350.023.81787533
CTCGTAT49250.022.70537444
ACGTGAA54300.022.41641832
TGAAACG56650.021.248935
GTCACGT58250.021.09060529
CACGTGA59100.020.86314231
AACGATC54000.020.41659438
CACACGT66000.019.2604312
ATCGGAA64450.019.2403012
TCGGAAG65850.019.138013
AAACGAT63250.018.74703837
CACGTCT67250.018.73515114
TCACGTG66450.018.31859430
GAAACGA70850.017.11715136
ACACGTC83500.016.16685113
CAGTCAC76950.016.08217427
Produced by FastQC (version 0.11.5)