FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n01_N2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n01_N2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27167745
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272040.10013344869071762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG38800.024.87655315
AGTCACG38700.024.53540828
CGTCTGA39850.024.05170816
ACGTCCG41450.022.20106132
CTCGTAT41650.021.23059544
GTCCGCA44750.020.56423234
TCGTATG44050.020.02279745
GTCACGT52400.017.99179629
CCGCACA54150.017.49299836
CACGTCT59250.016.82196814
TCGGAAG58200.016.8194433
ATCGGAA57650.016.7463172
TCCGCAC58000.016.33179535
CACACGT62100.016.01380312
TCACGTC59050.015.88935830
CAGTCAC70400.014.12669627
ACACGTC73900.014.06562113
CGCACAT70000.013.27491837
TCTCGTA69350.012.97771243
CCAGTCA79900.012.47521226
Produced by FastQC (version 0.11.5)