FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n01_N2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n01_N2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29211793
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG46750.024.64072415
CGTCTGA50950.022.565316
GTCCGCA52100.021.50666636
CTCGTAT48200.019.74549944
ACCCGTC60300.019.06659732
TCGTATG49750.018.1805845
ATCGGAA66600.017.4361212
TCGGAAG67200.017.179243
CACGTCT68500.017.14518214
CGCATCT64000.016.94524239
CACACGT70900.016.53316112
TCCGCAT68350.016.42644337
TCACCCG72350.015.611999530
CAGTCAC79650.014.66140827
CCCGTCC80150.014.62549233
GTCACCC79300.014.555929
CCGTCCG85400.013.99003734
ACACGTC87650.013.861299513
AGTCACC88100.013.66383928
CGTCCGC92750.013.17247435
Produced by FastQC (version 0.11.5)