FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n01_N2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n01_N2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24009733
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG37800.024.04650315
CGTCTGA41250.022.25356116
CTCGTAT30400.017.54099844
ATCGGAA53900.017.118192
TCGTATG29800.016.9881445
CACACGT55300.016.96565212
CACGTCT56700.016.5466114
TCGGAAG58150.016.0988433
GTCAGAA58800.015.0002136
CAGTCAC63550.014.51657427
TGTCAGA63350.014.1714435
ACACGTC72000.013.74913313
AGTCACA67350.013.56389128
GTCACAT69750.013.03265929
CCAGTCA77900.011.92912126
TCACATG78250.011.8181830
GATCGGA79050.011.7863011
ACATGTC77650.011.73492932
AATCTCG60700.011.63917941
CACATGT79850.011.60938331
Produced by FastQC (version 0.11.5)