FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n01_N2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n01_N2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35324297
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG1118410.316612104127649TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG177500.035.73419634
TCCGTAT175600.035.4287737
CGTATCT174050.035.26601439
CCGTATC177050.034.9987738
TCGTATG150450.034.94959345
CTCGTAT160550.033.91412744
GTTCCGT190300.032.9874735
TTCCGTA190500.032.81124536
ACGTCTG199400.032.38317515
CGTCTGA200200.032.2087716
TATCTCG187200.031.33385741
CACGTCT226700.028.68189214
CACACGT230700.028.16508312
GTATCTC220700.027.92340540
TCGGAAG234400.027.6592223
TCTCGTA199000.027.55358143
GTCACAG232100.027.53204329
TCACAGT232250.027.51414530
ATCGGAA235750.027.4921042
CAGTCAC233100.027.48138427
Produced by FastQC (version 0.11.5)