FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l02_n01_N2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l02_n01_N2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25992434
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269980.10386868732647356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG53350.027.49673515
CGTCTGA55650.026.44116216
CTCGTAT51800.024.71489744
TCGTATG53250.024.16866945
TCGGAAG69850.021.424473
ATCGGAA71050.020.9683692
CACGTCT72300.020.69420614
CACAGTC72750.020.07230431
AGTCAAC73350.019.9079234
CACACGT75650.019.7779612
AATCTCG74400.018.78004541
CAGTCAC79550.018.72434427
ACACGTC84200.018.14358513
GTCACAG82500.018.0820829
TCACAGT84900.017.2793730
AGTCACA85500.017.15814428
TCTCGTA80350.016.26921543
ACAGTCA97550.015.68404132
GATCGGA97350.015.6278121
CCAGTCA96150.015.49164526
Produced by FastQC (version 0.11.5)