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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-04-17, 15:04 based on data in: /beegfs/mk5636/logs/html/CCWEBACXX/1

        General Statistics

        Showing 20/20 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        CCWEBACXX_l01_n01_P2W1
        70.0%
        52%
        29.5
        CCWEBACXX_l01_n01_P2W2
        73.1%
        51%
        42.2
        CCWEBACXX_l01_n01_P2W3
        71.0%
        52%
        32.2
        CCWEBACXX_l01_n01_P2W4
        68.6%
        50%
        30.0
        CCWEBACXX_l01_n01_P2W5
        70.4%
        52%
        29.7
        CCWEBACXX_l01_n01_P2W6
        69.9%
        51%
        31.2
        CCWEBACXX_l01_n01_P2W7
        67.1%
        51%
        28.6
        CCWEBACXX_l01_n01_P2W8
        68.1%
        51%
        31.9
        CCWEBACXX_l01_n01_P2W9
        71.1%
        52%
        29.7
        CCWEBACXX_l01_n01_undetermined
        91.4%
        45%
        14.1
        CCWEBACXX_l01_n02_P2W1
        67.4%
        54%
        29.5
        CCWEBACXX_l01_n02_P2W2
        70.8%
        53%
        42.2
        CCWEBACXX_l01_n02_P2W3
        67.8%
        54%
        32.2
        CCWEBACXX_l01_n02_P2W4
        67.3%
        53%
        30.0
        CCWEBACXX_l01_n02_P2W5
        67.2%
        55%
        29.7
        CCWEBACXX_l01_n02_P2W6
        68.0%
        54%
        31.2
        CCWEBACXX_l01_n02_P2W7
        63.9%
        54%
        28.6
        CCWEBACXX_l01_n02_P2W8
        66.3%
        53%
        31.9
        CCWEBACXX_l01_n02_P2W9
        68.4%
        54%
        29.7
        CCWEBACXX_l01_n02_undetermined
        89.0%
        45%
        14.1

        Lane 1 Demultiplexing Report

        Lane 1 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 10/10 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        14,065,749
        4.7
        P2W1
        29,492,092
        9.9
        P2W2
        42,228,683
        14.1
        P2W3
        32,204,607
        10.8
        P2W4
        30,047,914
        10.0
        P2W5
        29,667,079
        9.9
        P2W6
        31,212,257
        10.4
        P2W7
        28,622,528
        9.6
        P2W8
        31,899,421
        10.7
        P2W9
        29,728,122
        9.9

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        TAAGTT
        152354.0
        1.1
        TAAGGT
        130855.0
        0.9
        TAACGT
        103461.0
        0.7
        TAACTT
        97322.0
        0.7
        CAAGTT
        83727.0
        0.6
        CAAGGT
        77712.0
        0.6
        CAACGT
        70576.0
        0.5
        CCCCGC
        69663.0
        0.5
        TAAGGG
        68608.0
        0.5
        TCAGTT
        67902.0
        0.5
        TACCGT
        67454.0
        0.5
        TAAGTG
        66190.0
        0.5
        TAAATT
        66098.0
        0.5
        TAAAGT
        64671.0
        0.5
        CAACTT
        59971.0
        0.4
        GTTCGG
        59678.0
        0.4
        CAAGGG
        58889.0
        0.4
        CCCCCC
        58469.0
        0.4
        TACCTT
        58463.0
        0.4
        TACGTT
        57884.0
        0.4

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        1.0
        327,232,192
        299,168,452
        4.7
        5.0

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (51bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        20 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.