FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14065749
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC3950.026.77313232
GAGTGTA4150.023.85374828
CACTCTT7900.023.0781842
TAGATCT4600.022.986733
AGTGTAG4350.022.75977929
GTGTAGA4700.022.02208330
TCTCGGT4700.021.54410737
TGTAGAT5650.018.3191931
TAGGGAA5950.018.1480520
TGTAGGG6500.017.30463218
GTAGGGA6400.017.22345519
GATCTCG7150.016.9898235
GTGTAGG6500.016.95847917
TCGTGTA6450.016.39361615
CTCGGTG7100.015.53150138
TCGGTGG7300.015.1072739
TCGGAAG11250.014.8051253
ATCGGAA11300.014.1424182
AGATCTC8000.014.05896234
TTCCCTA4150.013.5511448
Produced by FastQC (version 0.11.5)