FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n02_P2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n02_P2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29728122
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28400.011.8090432
GTAGAAC35550.010.37778711
GTACTCG54900.010.2074734
GCGTGAC41200.09.4544761
TACTCGA58150.09.4423685
CACTCGT61300.09.39485835
ACTCGTG64050.09.09763736
TCACTCG65000.08.89436934
AGTACTC65850.08.8867093
AATCGAA58500.08.8858085
GCGGAAA77350.08.8491571
TCAGGTA45400.08.8199797
GGAAAAT119500.08.6483531
GAATCGA46800.08.55983454
CTCTATA71400.08.54103345
CTCGAAT30000.08.4809761
GGTAGAA47300.08.37054110
AAAATCC87750.08.3103883
TAGAACA49050.07.705083412
CGCGAAT30200.07.67514241
Produced by FastQC (version 0.11.5)