FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n02_P2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n02_P2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31899421
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGA45200.010.9539764
CTAATCG38450.010.6536563
GTAGAAC55000.09.77528211
AATCGAG66250.09.7146725
AATCGAA65150.09.0497145
CTCGAAT37850.08.9230751
CAGGTAG61350.08.4333728
TCAGGTA67150.08.3081547
GAATCGA52350.08.2111544
GGAAAAT153850.07.97605561
GGGAAAT83000.07.89412641
GGTAGAA68300.07.805926310
TAGAACA69600.07.724877412
AAAATCC111350.07.70119953
AGGTAGA71600.07.50896939
CACGAAG68200.07.3952491
CGAATCG38500.07.30756863
GCTAATC68800.07.1648182
GAAAAAT210300.07.08774231
AAAAATC107450.07.0804322
Produced by FastQC (version 0.11.5)