FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n02_P2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n02_P2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28622528
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC39850.010.72569811
CGTGACT21950.010.1517842
CTCGAAT31100.09.2667931
TCAGGTA51350.08.8932187
GAATCGA48300.08.8529694
GGTAGAA50450.08.60593210
CAGGTAG52150.08.4546588
AATCGAA51200.08.2187025
TAGAACA53850.08.02096212
GCGGAAA70050.07.93905261
GCGTGAC33200.07.79901271
GAATTGA78650.07.7544864
CACTCGT51600.07.629729735
GTACTCG49100.07.6086754
AAAAATC88700.07.435082
ACTCGTG55250.07.41119136
AACGGCC118250.07.3834985
GGAAAAT112500.07.2849861
TACTCGA51150.07.2588855
AAAATCC80100.07.24944263
Produced by FastQC (version 0.11.5)