FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n02_P2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n02_P2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31212257
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26100.011.3835112
GTAGAAC44400.011.2984911
TCAGGTA54000.09.7898197
GAATCGA54750.09.7009674
CACTCGT60850.09.39053635
CTCGAAT35450.09.0190571
AATCGAA61750.09.0013025
GTACTCG55600.08.9454994
CAGGTAG57600.08.9434248
ACTCGTG66450.08.83683836
TACTCGA57750.08.8064695
CTCTATA68300.08.5988345
GCGGAAA80800.08.5270561
GGTAGAA57800.08.52342310
TCACTCG68100.08.52270334
GGAAAAT123700.08.2273081
TAGAACA61300.08.1470712
GCGTGAC41800.08.0798641
GAATTGA81800.08.00620654
TCGTGAG75350.07.91403938
Produced by FastQC (version 0.11.5)