FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n02_P2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n02_P2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29667079
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27550.013.4805162
GTAGAAC41050.011.7821411
GCGTGAC38850.010.5481121
GTACTCG65200.010.2172344
CTCGAAT30300.010.1805741
TCAGGTA51950.010.132937
TACTCGA66550.09.9414165
AGTACTC75750.09.2108683
TAGAACA52600.09.10966112
GGAAAAT124150.08.94117451
GGTAGAA55400.08.77091810
GAATCGA49600.08.5303224
GTGACTC47450.08.3957313
CAGGTAG61150.08.3875488
AATCGAA58050.08.3346085
AAAATCC95450.07.97007233
GAATTGA77450.07.93287234
CACTCGA32350.07.9308514
TATAAGG30700.07.84321985
CTATAAG29750.07.71618184
Produced by FastQC (version 0.11.5)