FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n02_P2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n02_P2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30047914
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25600.09.7598112
GTAGAAC36200.08.76212311
GGAAAAT122500.08.4368931
GGACTTA43300.08.1604434
CACTCGT72000.07.99872435
AATCGAA65150.07.94462635
TCAGGTA46500.07.88569557
AAAATCC90500.07.8590513
GAATCGA48850.07.60188634
CTCTATA78300.07.587460545
ACTCGTG73400.07.50992136
GCGGAAA91650.07.3458951
GAATTGA86250.07.3063574
CTCGAAT31550.07.27958771
TCACTCG77000.07.274426534
GCGTGAC36900.07.26150371
AGAACGG167750.07.20515633
AAAAATC91250.07.1289142
GAAAAAT167750.07.1006671
GACTTAT48850.07.04834035
Produced by FastQC (version 0.11.5)