FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n02_P2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n02_P2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32204607
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT31600.010.7559612
GTACTCG69400.09.7936544
CTCGAAT35000.09.4570961
GTAGAAC42150.09.44656711
TACTCGA70800.09.3447035
GAATCGA53700.09.0945834
GCGTGAC42500.09.0067591
GGAAAAT133050.08.8679851
AGTACTC82750.08.7583083
AATCGAA63300.08.3544065
AAAATCC96050.08.2251183
CACTCGT59050.08.11475835
TCAGGTA52650.08.075577
GAATTGA87200.08.0009674
CTCTATA63050.07.851974545
ATGTCTC133000.07.783020543
TCACTCG61950.07.625509734
TGTCTCC136900.07.594161544
GAAAAAT160500.07.56174951
ACTCGTG64250.07.528833436
Produced by FastQC (version 0.11.5)