FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n02_P2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n02_P2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42228683
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT38900.011.5148872
GTAGAAC53450.09.09091411
GCGTGAC53900.09.0650191
AATCGAA82200.08.48681455
GGAAAAT182800.08.4005151
GAATTGA122400.08.3661464
GAATCGA67000.08.2633434
AAAATCC127200.08.2457143
CTCGAAT44450.08.1555231
TCAGGTA71000.07.9527237
GTACTCG76750.07.74142654
AGAACGG244000.07.4441163
ATGTCTC183100.07.423118643
AATTGAC78700.07.377345
TACTCGA80700.07.27816345
GATTCGA187650.07.21675134
TGTCTCC188200.07.12629844
CGTTTAA226050.07.116354533
GTGACTC72750.07.11580853
AAATCCT141250.07.07440574
Produced by FastQC (version 0.11.5)