FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n02_P2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n02_P2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29492092
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27050.012.2321882
GTAGAAC35700.09.26292911
GTACTCG54050.09.1605794
GCGTGAC39700.09.0744731
CTCGAAT32500.08.9371351
TACTCGA55600.08.7423855
GAATTGA82350.08.5814754
AATCGAA57650.08.5095815
TCAGGTA46850.08.1628997
GAATCGA47200.07.96288444
GGAAAAT129100.07.90066961
AATTGAC55400.07.8803045
AGAACGG166200.07.35361433
CGGGTAT12950.07.30249831
AAAATCC91950.07.2700393
TGTCTCC112600.07.254376444
AAAAATC82900.07.19524342
CACTCGT53650.07.170349635
AGTACTC72200.07.17005833
GTGACTC50950.07.1565363
Produced by FastQC (version 0.11.5)