FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14065749
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA8350.035.26560616
ACGTCTG8700.033.3300115
CACACGT8950.032.3973912
CACGTCT9900.029.28844314
ACACGTC10600.027.35430313
AGCACAC11600.025.18716610
CAGTCAC8050.023.7976327
GCACACG12650.023.4545311
TCGGAAG15700.023.3527013
ATCGGAA15800.023.2054772
CCAGTCA9000.023.03859326
CGGAAGA16850.022.0214884
CCGAGAC4700.021.57831629
GATCGGA17250.021.2557031
GAGACCG5000.019.82022531
CTCCAGT12750.019.44426724
AACTCCA14750.019.38638322
AGACCGA5150.018.80432332
GAACTCC16450.018.73311621
ACCGATC4950.018.17455334
Produced by FastQC (version 0.11.5)