FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n01_P2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n01_P2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29728122
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401930.1352019478391538No Hit
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA382060.1285180409310753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG59800.030.7758415
CGTAATC58200.029.46035838
CCGTACG60050.029.33924533
CGTCTGA62600.029.2917616
CTCGTAT54000.026.4190344
ACCGTAC72250.025.03642732
CACCGTA70300.024.89888631
TCGTATG55200.023.60269545
ATCGGAA77600.023.4901392
TCGGAAG79000.023.4436263
CACGTCT83050.022.701914
GTAATCT77200.022.44292439
CACACGT86350.021.52164812
CAGTCAC89250.020.16695427
ACGTAAT89650.019.10034237
TAATCTC92000.018.63766340
TACGTAA93550.018.59257536
AATCTCG88650.018.32663541
AGTCACC99450.018.32478128
ACACGTC106000.017.99897813
Produced by FastQC (version 0.11.5)