FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n01_P2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n01_P2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31899421
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343560.10770101438518273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG44200.030.44260233
ACGTCTG51300.026.92809115
AGTCACG51800.026.36433828
GTTTCGG53800.025.17815634
GTCACGT58450.023.1337429
TTCGGAA57950.023.10325636
CGTCTGA59950.023.0054816
ACGTTTC61100.022.35097932
TTTCGGA61250.021.89524735
TCGGAAT66600.019.76518237
CACACGT70600.019.75793312
TCGGAAG73000.019.0200713
ATCGGAA72400.018.9607472
CTCGTAT46400.018.18594744
CACGTCT82050.017.32977914
CGGAATC77950.016.91611938
ACACGTC86700.016.65982613
CACGTTT84100.016.26514831
TCGTATG50650.015.72704345
GGAATCT81900.015.71561439
Produced by FastQC (version 0.11.5)