FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n01_P2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n01_P2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28622528
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA333620.116558537387054No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG323000.11284817329901818TruSeq Adapter, Index 20 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300840.10510601998537657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG67200.031.8063728
ACGTCTG69150.031.39715815
CGTCTGA71550.030.50149316
GGCCTTA72550.028.84383836
CACGTGG73950.028.50733631
GTCACGT78500.027.02710529
CTCGTAT61700.026.07652344
TCGTATG57500.025.94633545
GCCTTAT81200.025.74352637
CACGTCT86900.025.39828914
CACACGT86000.025.32398812
ATCGGAA86050.025.3152872
TCGGAAG87600.025.0207963
ACGTGGC87400.023.96588532
TATCTCG78750.023.4311141
TCACGTG93100.022.93369330
CAGTCAC94800.022.83108127
CCTTATC94350.021.96473938
CTTATCT94500.021.52508739
ACACGTC109250.020.73782513
Produced by FastQC (version 0.11.5)