FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n01_P2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n01_P2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31212257
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398150.1275620664023111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG49000.025.43703815
AGTCACG49700.024.76229128
CGTCTGA51900.024.0160116
CTCGTAT47350.023.14414644
CGTGAAA52450.023.1228533
TCGTATG50100.022.7721445
ACGTGAA58050.021.23879432
CACGTGA58550.021.13437531
GTCACGT61750.019.82079529
TGAAACG61400.019.7525335
AACGATC56950.019.5972538
TCGGAAG66350.019.1966343
ATCGGAA66250.019.0901072
CACGTCT69650.018.05691714
CACACGT73800.017.37686312
AAACGAT69450.017.10680237
GAAACGA76950.015.93639836
CAGTCAC83750.015.44695427
TCACGTG81500.015.2660530
ACACGTC91300.014.48970213
Produced by FastQC (version 0.11.5)