FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n01_P2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n01_P2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29667079
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG840700.28337808383494717TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG133850.038.9128915
AGTCACG132900.038.31086328
CGTCTGA138800.037.55769316
ACGTCCG138650.036.57539732
GTCCGCA137650.036.5348734
CTCGTAT125100.035.81209644
TCGTATG121350.035.23143445
GTCACGT148400.034.44566329
ATCGGAA152250.034.054852
CCGCACA146350.034.00937736
TCGGAAG154000.033.8424223
TCCGCAC150450.033.59107235
CACGTCT156650.033.47902314
CACACGT157750.033.25983412
TCACGTC160500.031.82073830
CAGTCAC165100.031.152427
CGCACAT163200.030.04330637
ACACGTC177700.029.80436713
TCTCGTA155100.029.21916643
CGTCCGC178200.028.53638533
Produced by FastQC (version 0.11.5)