FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n01_P2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n01_P2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30047914
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG431210.14350746610896184TruSeq Adapter, Index 16 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318760.10608390319540983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG97450.033.61527315
CGTCTGA99450.032.91689716
GTCCGCA101850.030.75211736
CTCGTAT76500.028.88489344
TCGGAAG115050.028.4598433
ATCGGAA115400.028.3744262
ACCCGTC111700.028.29945832
CACGTCT117300.028.04183814
TCACCCG115600.027.61746630
TCGTATG74500.027.60654645
CACACGT123000.027.01671212
TCCGCAT114500.026.68686937
CGCATCT110350.026.65057839
CCCGTCC129150.024.84390333
CAGTCAC130200.024.74517827
ACACGTC134600.024.62151713
CCGTCCG132650.024.27336334
AGTCACC134900.024.21660428
CGTCCGC133700.024.18367235
GTCACCC133450.024.07510629
Produced by FastQC (version 0.11.5)