FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n01_P2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n01_P2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32204607
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA461030.1431565365787572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG51900.025.05632615
CGTCTGA53650.024.36501516
CACGTCT72200.018.60347414
TCGGAAG72000.018.4403633
ATCGGAA71400.018.4380822
CACACGT75350.017.85561612
GTCAGAA79250.016.1838236
TGTCAGA81400.016.08807435
CAGTCAC86900.015.22359327
CTCGTAT39200.015.0396944
GTCACAT87600.015.02486929
AGTCACA88100.014.86300728
ACACGTC100250.014.16125913
TCGTATG38900.013.94092945
CCAGTCA102250.012.982226
TCACATG104350.012.89338630
ACATGTC104050.012.82226632
TCCAGTC108000.012.24933525
CGGAAGA113250.012.2188644
CACATGT111200.012.11926531
Produced by FastQC (version 0.11.5)