FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n01_P2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n01_P2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42228683
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG1597440.378283168338449TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT276500.037.9711239
AGTTCCG294100.037.91713334
TCCGTAT288100.037.65283637
CCGTATC286850.037.63649738
TCGTATG213750.036.68809545
CTCGTAT230550.036.2692544
GTTCCGT306000.035.98667535
TTCCGTA305550.035.7524836
ACGTCTG323600.035.180315
CGTCTGA323250.035.16991816
TATCTCG288050.032.88870641
CACGTCT353350.032.3138314
CACACGT355900.032.05702212
GTATCTC324300.031.97408940
GTCACAG349500.031.88439429
ATCGGAA361200.031.500892
CAGTCAC356200.031.49324627
TCACAGT352550.031.48722830
TCGGAAG363400.031.3590953
ACAGTTC356400.031.35518632
Produced by FastQC (version 0.11.5)