FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l01_n01_P2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l01_n01_P2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29492092
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377720.1280750107520348No Hit
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA327540.11106028015916945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG56600.029.49490715
CGTCTGA59750.028.01544616
CTCGTAT52900.024.92687244
TCGTATG53000.024.41289345
ATCGGAA73700.022.717782
TCGGAAG75350.022.369263
CACGTCT76150.022.2181314
CACACGT76850.022.07430512
CACAGTC76550.021.77843531
AGTCAAC78450.020.99507534
CAGTCAC86750.019.45142227
GTCACAG87700.019.08669529
AGTCACA89500.018.75319928
TCACAGT91200.018.47754930
ACACGTC96950.018.1475713
AATCTCG84250.017.5746941
TCTCGTA83250.016.46132543
ACAGTCA102650.016.43823832
CCAGTCA102500.016.33084326
GATCGGA107350.015.7648641
Produced by FastQC (version 0.11.5)