FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19071788
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG9600.018.75360329
CACTCTT15600.017.6093792
GTAGATC11000.017.18336332
TCTCGGT11250.016.9975637
TAGATCT11350.016.65029133
GAGTGTA10950.016.44143128
GTGTAGA11400.015.98911930
TCGTGTA12750.015.1728615
TCCCTAC8650.014.82535659
GTGTAGG13300.014.54571617
GTAGGGA13450.014.04973319
TTCCCTA10300.013.9794218
TGTAGGG13450.013.88116418
TAGGGAA14350.013.01193320
CTCGGTG17550.012.81853838
TGTAGAT14250.012.79075831
TACACGA7850.012.32197313
GATCTCG17800.011.62690435
TCGGTGG19000.010.53782339
GTACTCG8650.010.4107234
Produced by FastQC (version 0.11.5)