FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n02_S2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n02_S2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25142216
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC42600.012.463511
TCAGGTA56400.010.2922267
TAGAACA55800.09.5951712
GGAAAAT138550.09.5399111
AAAATCC101150.09.4156833
TAATCGA27300.09.1538164
CATAAGC96150.09.0090737
CAGGTAG62450.09.0067038
GGTAGAA61400.08.97733410
AGTGTGA65800.08.75160514
AGCATAA93050.08.6859675
GTGTGAG65100.08.63842115
GTACTCG46150.08.5858414
AAATCCT103450.08.4656624
AGGTAGA67300.08.4244779
GCATAGG35300.08.41182341
AATCGAG46100.08.39977555
AATCCTT88200.08.2957355
AATCGAA57700.08.2327845
CGCGATA11000.08.18315329
Produced by FastQC (version 0.11.5)