FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n02_S2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n02_S2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25483476
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG57700.013.3441034
TACTCGA64900.011.8289745
GTAGAAC35800.011.68872411
AGTACTC69700.011.5323333
AATCGAA44600.09.287965
AAAATCC85400.09.0694793
GGAAAAT114550.09.0618441
TCAGGTA52250.08.9997667
CGTGACT22950.08.8302362
TAGAACA48750.08.76779812
ACACGCG27400.08.6220746
CTCGAAT21050.08.4505651
GGTAGAA50650.08.39520810
ACTCGAG101050.08.2828466
GAATCGA35650.08.2727694
AATTGAC42100.08.181765
AATCCTT76050.08.0520615
TTACGAC26900.07.94748229
AGTGTGA55500.07.90346314
TCACACG23800.07.84477315
Produced by FastQC (version 0.11.5)