FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l08_n02_S2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l08_n02_S2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20298362
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG31900.010.1622774
GTAGAAC28600.09.91151311
AGTACTC42850.08.5645223
TACTCGA39050.08.4168585
AATCGAA39750.07.81555945
TCAGGTA39200.07.7486117
CACTCGT48900.07.728652535
CGTGACT20700.07.72293142
CTCTATA49300.07.711138745
GGAAAAT81900.07.61537171
GAATCGA31350.07.6118024
AAAAATC62850.07.4874712
CGTATAG12650.07.469215412
CGGAAAT17500.07.4625151
CGCGATA9700.07.424016529
CTCGAAT17750.07.3574091
ACTCGAG52700.07.34338246
GAATTGA49600.07.30740834
GCGGAAA58550.07.30671071
TAGAACA40150.07.228037412
Produced by FastQC (version 0.11.5)